Summary
Type: TLC
Application #: 400320
Catalog#: 1824126 - Silica N HR TLC Plates, w/UV254, polyester backed, 200um, 20x20cm, 25/pk
Detection: A) UV 254 nm
B) conc. sulphuric acid, then UV
C) 38% aqueous formaldehyde solution in conc. sulphuric acid 1:10, then UV,
D) ammonium metavanadate in conc. sulphuric acid (see chapter visualization
Conditions: Eluent:
ethyl acetate / methanol (40:5, v/v) 5 cm ascending (eluent I), then view under UV, then 2nd development with eluent II (ethyl acetate / methanol / aqueous ammonia solution d=0.88, 40:5:5, v/v), 8 cm ascending
Authors: Jack, D.
Source: J. Chromatogr. 187 (1980) 277